Kategori: science
Preventing Medical Mistakes
Medical mistakes continue to be a significant cause of preventable deaths within the United States. While death is the most tragic outcome, medical mistakes cause other problems such as permanent disabilities, extended hospital stays, longer recoveries, and even additional treatments. Medical Mistakes Medical mistakes and their consequences also add significantly to the overall cost of […]
Overview of real-time PCR components
This section provides an overview of the major reaction components and parameters involved in real-time PCR experiments. A more detailed discussion of specific components like reporter dyes, passive reference dyes, and uracil DNA glycosylase (UDG) is provided in subsequent sections of this handbook. DNA polymerase PCR performance is often related to the thermostable DNA polymerase, […]
ELISA Equipment, Maintenance and Calibration
Equipment for ELISA testing is widely available. Readers, washers, and pipettes are available as manual or automated systems. Some of the factors affecting equipment selection are the number and types of tests and samples, technical training of staff, and financial considerations. Below is a brief outline of some equipment available for performing ELISA testing. Pipettes […]
Indirect ELISA – Antigen Coating, Blocking, Preparation
Indirect ELISA – This is a general protocol in which antigen coating and blocking may not be required if the wells from the manufacturer have been pre-adsorbed with the antigen. 1. Antigen Coating Dilute purified antigens to a final concentration of 1-10 μg/mL in bicarbonate/carbonate antigen-coating buffer (100 mM NaHCO3 in deionized water; pH adjusted […]
Competitive ELISA : Antigen Coating, Blocking, Preparation
Competitive ELISA, This is a general protocol in which antigen coating and blocking may not be required if the wells from the manufacturer have been pre-adsorbed with the antigen. 1. Antigen Coating Dilute purified antigens to a final concentration of 20 μg/ml in bicarbonate/carbonate antigen-coating buffer (100 mM NaHCO3 in deionized water; pH adjusted to […]