Tag: laboratory
Sandwich ELISA – Antibody Coating, Blocking, Preparation
Sandwich ELISA – All of the ELISA kits from Boster use the sandwich format and avidin-biotin chemistry. Our ELISA assays require the dilutions of standard solutions, biotinylated antibody (detection antibody) and avidin-biotinperoxidase. 1. Capture Antibody Coating (These steps are not required if the pre-adsorbed Picokine ELISA kits from Boster are used) Dilute the capture antibody […]
Competitive ELISA : Antigen Coating, Blocking, Preparation
Competitive ELISA, This is a general protocol in which antigen coating and blocking may not be required if the wells from the manufacturer have been pre-adsorbed with the antigen. 1. Antigen Coating Dilute purified antigens to a final concentration of 20 μg/ml in bicarbonate/carbonate antigen-coating buffer (100 mM NaHCO3 in deionized water; pH adjusted to […]
ELISA Test (enzyme-linked immunosorbent assay)
ELISA Test – ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity […]