Tag: PCR

  • Overview of real-time PCR components

    This section provides an overview of the major reaction components and parameters involved in real-time PCR experiments. A more detailed discussion of specific components like reporter dyes, passive reference dyes, and uracil DNA glycosylase (UDG) is provided in subsequent sections of this handbook. DNA polymerase PCR performance is often related to the thermostable DNA polymerase, […]